14WD

Crystal Structure of FtsZ with F6617-2523

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.730, 88.730, 179.617
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.404 - 1.926
Rwork	0.181
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.740
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.365	1.959
High resolution limit [Å]	1.926	1.926
Rmerge	0.180	2.274
Rmeas	0.188	2.364
Rpim	0.051	0.644
Total number of observations	805242	40031
Number of reflections	60302	2976
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.998	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-41	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.