14WC

Crystal Structure of FtsZ with Z33452106

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.595, 88.595, 179.328
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.337 - 1.973
Rwork	0.182
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.744
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.298	2.007
High resolution limit [Å]	1.973	1.973
Rmerge	0.201	2.653
Rmeas	0.209	2.760
Rpim	0.057	0.759
Total number of observations	743577	36841
Number of reflections	55831	2772
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	13.3	13.3
CC(1/2)	0.998	0.332

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-408	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.