14WB

Crystal Structure of FtsZ with Z111782404

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.627, 88.627, 179.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.054 - 2.444
Rwork	0.174
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.537
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.017	2.486
High resolution limit [Å]	2.444	2.444
Rmerge	0.255	2.589
Rmeas	0.265	2.689
Rpim	0.072	0.724
Total number of observations	397137	19848
Number of reflections	29270	1444
<I/σ(I)>	7
Completeness [%]	99.4
Redundancy	13.6	13.7
CC(1/2)	0.997	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-407	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.