14VZ

Crystal Structure of FtsZ with Z975817026

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.463, 88.463, 179.213
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.980 - 2.178
Rwork	0.192
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.588
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.943	2.215
High resolution limit [Å]	2.178	2.178
Rmerge	0.340	3.250
Rmeas	0.354	3.377
Rpim	0.097	0.917
Total number of observations	555376	28683
Number of reflections	41444	2112
<I/σ(I)>	6.4
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.994	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-400	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.