14VX

Crystal Structure of FtsZ with Z1551999220

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.718, 88.718, 180.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.845 - 2.357
Rwork	0.175
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.585
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.813	2.398
High resolution limit [Å]	2.357	2.357
Rmerge	0.378	2.984
Rmeas	0.393	3.114
Rpim	0.107	0.887
Total number of observations	441268	21048
Number of reflections	32891	1702
<I/σ(I)>	5.4
Completeness [%]	99.3
Redundancy	13.4	12.4
CC(1/2)	0.994	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-389	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.