14VW

Crystal Structure of FtsZ with Z385450668

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	89.002, 89.002, 181.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.541 - 2.287
Rwork	0.181
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.479
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.501	2.327
High resolution limit [Å]	2.287	2.287
Rmerge	0.267	3.082
Rmeas	0.277	3.206
Rpim	0.076	0.878
Total number of observations	493295	24043
Number of reflections	36596	1806
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	13.5	13.3
CC(1/2)	0.996	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-387	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.