14VQ

Crystal Structure of FtsZ with Z104492884

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.679, 88.679, 178.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.379 - 2.182
Rwork	0.179
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.696
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.340	2.220
High resolution limit [Å]	2.182	2.182
Rmerge	0.416	3.611
Rmeas	0.433	3.752
Rpim	0.118	1.017
Total number of observations	555450	28790
Number of reflections	41234	2110
<I/σ(I)>	6.3
Completeness [%]	100.0
Redundancy	13.5	13.6
CC(1/2)	0.992	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-321	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.