14VO

Crystal Structure of FtsZ with Z19733482

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.568, 88.568, 178.797
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.323 - 2.168
Rwork	0.177
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.593
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.284	2.206
High resolution limit [Å]	2.168	2.168
Rmerge	0.285	2.446
Rmeas	0.296	2.541
Rpim	0.080	0.686
Total number of observations	563843	28301
Number of reflections	41951	2077
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.995	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-318	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.