14VN

Crystal Structure of FtsZ with Z26781964

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.100, 88.100, 176.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.089 - 2.205
Rwork	0.169
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.611
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	2.243
High resolution limit [Å]	2.205	2.205
Rmerge	0.259	2.507
Rmeas	0.269	2.605
Rpim	0.073	0.705
Total number of observations	523830	25974
Number of reflections	38935	1917
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	13.5	13.5
CC(1/2)	0.996	0.313

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-308	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.