14VM

Crystal Structure of FtsZ with F2189-0254

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.277, 88.277, 178.366
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.177 - 1.820
Rwork	0.180
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.788
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.139	1.852
High resolution limit [Å]	1.820	1.820
Rmerge	0.160	2.884
Rmeas	0.166	2.997
Rpim	0.046	0.810
Total number of observations	853197	47344
Number of reflections	68521	3483
<I/σ(I)>	11.2
Completeness [%]	97.6
Redundancy	12.5	13.6
CC(1/2)	0.998	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-302	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.