14VL

Crystal Structure of FtsZ with F6608-1013

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.092, 88.092, 177.336
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.085 - 1.934
Rwork	0.177
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.552
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.046	1.967
High resolution limit [Å]	1.934	1.934
Rmerge	0.187	2.553
Rmeas	0.194	2.654
Rpim	0.053	0.724
Total number of observations	773818	38932
Number of reflections	57987	2902
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-301	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.