14VK

Crystal Structure of FtsZ with F6616-3495

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.868, 88.868, 179.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.474 - 2.108
Rwork	0.181
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.657
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.434	2.144
High resolution limit [Å]	2.108	2.108
Rmerge	0.281	2.634
Rmeas	0.292	2.747
Rpim	0.080	0.778
Total number of observations	619728	28377
Number of reflections	46168	2276
<I/σ(I)>	7.1
Completeness [%]	100.0
Redundancy	13.4	12.5
CC(1/2)	0.995	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-296	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.