14VI

Crystal Structure of FtsZ with F2111-0067

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.478, 88.478, 178.636
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.278 - 2.013
Rwork	0.179
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.695
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.239	2.047
High resolution limit [Å]	2.013	2.013
Rmerge	0.206	2.148
Rmeas	0.214	2.254
Rpim	0.060	0.665
Total number of observations	651892	27326
Number of reflections	52270	2577
<I/σ(I)>	9.7
Completeness [%]	100.0
Redundancy	12.5	10.6
CC(1/2)	0.997	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-292	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.