14VH

Crystal Structure of FtsZ with F2147-0796

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.947, 88.947, 179.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.513 - 1.977
Rwork	0.181
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.694
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.473	2.011
High resolution limit [Å]	1.977	1.977
Rmerge	0.198	2.530
Rmeas	0.206	2.630
Rpim	0.056	0.717
Total number of observations	749164	36822
Number of reflections	56119	2759
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	13.3	13.3
CC(1/2)	0.998	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-279	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.