14VG

Crystal Structure of FtsZ with F6546-1623

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.813, 88.813, 180.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.446 - 2.161
Rwork	0.175
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.828
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.407	2.198
High resolution limit [Å]	2.161	2.161
Rmerge	0.284	3.255
Rmeas	0.295	3.385
Rpim	0.082	0.928
Total number of observations	550849	27493
Number of reflections	42827	2094
<I/σ(I)>	7.8
Completeness [%]	99.8
Redundancy	12.9	13.1
CC(1/2)	0.996	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-268	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.