14VF

Crystal Structure of FtsZ with F2156-0066

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.690, 88.690, 179.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.384 - 2.127
Rwork	0.178
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.633
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.345	2.164
High resolution limit [Å]	2.127	2.127
Rmerge	0.260	2.312
Rmeas	0.270	2.406
Rpim	0.073	0.664
Total number of observations	602191	29229
Number of reflections	44771	2247
<I/σ(I)>	7.5
Completeness [%]	100.0
Redundancy	13.5	13
CC(1/2)	0.996	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-247	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.