14VE

Crystal Structure of FtsZ with F1294-0176

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.628, 88.628, 180.048
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.353 - 2.096
Rwork	0.183
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.678
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.314	2.132
High resolution limit [Å]	2.096	2.096
Rmerge	0.348	3.215
Rmeas	0.365	3.384
Rpim	0.107	1.048
Total number of observations	545921	24715
Number of reflections	46829	2334
<I/σ(I)>	6.4
Completeness [%]	100.0
Redundancy	11.7	10.6
CC(1/2)	0.993	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-242	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.