14VD

Crystal Structure of FtsZ with F6667-0580

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.715, 88.715, 180.552
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.397 - 1.921
Rwork	0.181
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.748
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.357	1.954
High resolution limit [Å]	1.921	1.921
Rmerge	0.175	3.014
Rmeas	0.182	3.133
Rpim	0.050	0.852
Total number of observations	815603	40711
Number of reflections	61073	3027
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.998	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-228	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.