14VA

Crystal Structure of FtsZ with F2683-0098

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.500, 88.500, 178.552
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.289 - 2.104
Rwork	0.177
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.681
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.250	2.140
High resolution limit [Å]	2.104	2.104
Rmerge	0.284	2.308
Rmeas	0.295	2.409
Rpim	0.080	0.687
Total number of observations	613654	27544
Number of reflections	45774	2250
<I/σ(I)>	7.2
Completeness [%]	100.0
Redundancy	13.4	12.2
CC(1/2)	0.992	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-221	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.