14UZ

Crystal Structure of FtsZ with F2101-0214

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.469, 88.469, 178.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.274 - 2.456
Rwork	0.196
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.566
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.235	2.498
High resolution limit [Å]	2.456	2.456
Rmerge	0.383	2.409
Rmeas	0.401	2.528
Rpim	0.115	0.724
Total number of observations	287057	12047
Number of reflections	27914	1346
<I/σ(I)>	6.9
Completeness [%]	96.8
Redundancy	10.3	9
CC(1/2)	0.973	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-219	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.