14UY

Crystal Structure of FtsZ with F1641-0016

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.568, 88.568, 178.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.323 - 2.087
Rwork	0.179
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.670
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.284	2.123
High resolution limit [Å]	2.087	2.087
Rmerge	0.282	2.279
Rmeas	0.293	2.380
Rpim	0.080	0.681
Total number of observations	626317	28540
Number of reflections	46908	2350
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	13.4	12.1
CC(1/2)	0.995	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-207	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.