14UW

Crystal Structure of FtsZ with F6666-3016

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.475, 88.475, 178.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.277 - 1.804
Rwork	0.183
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.737
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.237	1.835
High resolution limit [Å]	1.804	1.804
Rmerge	0.153	2.438
Rmeas	0.159	2.532
Rpim	0.043	0.680
Total number of observations	970293	49073
Number of reflections	72520	3607
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-189	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.