14UU

Crystal Structure of FtsZ with F6616-3157

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.675, 88.675, 179.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.377 - 1.992
Rwork	0.179
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.755
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.338	2.027
High resolution limit [Å]	1.992	1.992
Rmerge	0.219	2.098
Rmeas	0.228	2.182
Rpim	0.063	0.596
Total number of observations	722694	35378
Number of reflections	54286	2683
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	13.3	13.2
CC(1/2)	0.997	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-185	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.