14UR

Crystal Structure of FtsZ with F9995-1720

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.445, 88.445, 178.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.262 - 2.086
Rwork	0.180
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.660
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.222	2.122
High resolution limit [Å]	2.086	2.086
Rmerge	0.235	2.078
Rmeas	0.244	2.171
Rpim	0.068	0.623
Total number of observations	594196	28305
Number of reflections	45733	2337
<I/σ(I)>	9.3
Completeness [%]	97.5
Redundancy	13	12.1
CC(1/2)	0.997	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-158	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.