14GS
GLUTATHIONE S-TRANSFERASE P1-1 APO FORM 1
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.651, 91.826, 69.143 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gss |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.270 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.407 |
| Total number of observations | 17349 * | |
| Number of reflections | 10691 * | |
| <I/σ(I)> | 6.6 | 1.5 |
| Completeness [%] | 88.6 | 91.3 |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 22 * | pH 6.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | phosphate | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 5 | 1 | drop | adriamycin-GSH | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 28 (%(w/v)) | |
| 7 | 1 | reservoir | dithiothreitol | 65-75 (mM) | |
| 8 | 1 | reservoir | MES | 100 (mM) | |
| 9 | 1 | reservoir | dimethyl sulfoxide | 5 (%(v/v)) |
