13JQ

Structure of Thermus thermophilus Mfd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	97.026, 171.234, 177.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 2.600
R-factor	0.2287
Rwork	0.227
R-free	0.27160
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.660
High resolution limit [Å]	2.570	2.600
Rmeas	0.085
Number of reflections	45418	2296
<I/σ(I)>	22.3
Completeness [%]	96.0
Redundancy	12.3
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M KBr, 0.2 M KSCN, 34% PEG400, 0.1 M HEPES pH 7