13GO
Crystal Structure of Glycerol Dehydrogenase from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.502, 104.360, 104.258 |
| Unit cell angles | 90.00, 110.59, 90.00 |
Refinement procedure
| Resolution | 49.660 - 3.100 |
| R-factor | 0.2286 |
| Rwork | 0.225 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.660 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.241 | 0.885 |
| Rpim | 0.101 | 0.367 |
| Number of reflections | 27748 | 1332 |
| <I/σ(I)> | 5.6 | 2 |
| Completeness [%] | 99.2 | 96 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.953 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M Sodium Chloride, 0.1 M Imidazole:HCl, 1.0 M Ammonium Phosphate Dibasic |
