13DG

[2T7-22] 22 bp Tensegrity Triangle that Assembles into a Cubic Lattice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920119
Spacegroup name	P 41 3 2
Unit cell lengths	171.712, 171.712, 171.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.340 - 5.650
R-factor	0.1333
Rwork	0.130
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.414
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.342	34.342	5.904
High resolution limit [Å]	5.646	14.211	5.646
Number of reflections	2717	193	190
<I/σ(I)>	13.6
Completeness [%]	94.1
Redundancy	32.9
CC(1/2)	0.997	0.997	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate