13AF

Structure of R. denitrificans GapR bound to a 20 bp DNA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	36.861, 62.949, 83.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.430 - 2.970
R-factor	0.2529
Rwork	0.249
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.174
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.430	3.150
High resolution limit [Å]	2.970	2.970
Rpim	0.044	0.368
Number of reflections	2129	336
<I/σ(I)>	17.1	2.5
Completeness [%]	96.9
Redundancy	4.8
CC(1/2)	0.999	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35% Peg 400, 0.1 M CAPS pH 10.5