134L

ROLE OF ARG 115 IN THE CATALYTIC ACTION OF HUMAN LYSOZYME. X-RAY STRUCTURE OF HIS 115 AND GLU 115 MUTANTS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	58.620, 60.940, 30.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.770
R-factor	0.183
Rwork	0.183
RMSD bond length	0.013
RMSD bond angle	2.780
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.770 *
Rmerge	0.083 *
Total number of observations	34053 *
Number of reflections	9105 *
Completeness [%]	80.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium nitrate	7 (M)