12ZJ
Crystal structure of USP7 TRAF domain in complex with MAGEL2 peptide (968-980)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920208 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.483, 50.696, 82.974 |
| Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
| Resolution | 41.500 - 1.630 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 1.656 |
| High resolution limit [Å] | 1.628 | 1.628 |
| Rmerge | 0.112 | 1.588 |
| Rmeas | 0.145 | 2.082 |
| Rpim | 0.092 | 1.331 |
| Number of reflections | 58428 | 2903 |
| <I/σ(I)> | 6.4 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | 3.6 |
| CC(1/2) | 0.995 | 0.345 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289.15 | 250mM MgCl2 100mM Tris 30% PEG8000 |
