12NW

Crystal structure of a GH26 enzyme (EiGH26a) in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977200
Spacegroup name	C 1 2 1
Unit cell lengths	66.775, 65.328, 84.029
Unit cell angles	90.00, 110.39, 90.00

Refinement procedure

Resolution	45.200 - 1.530
R-factor	0.1876
Rwork	0.187
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.200	45.200	1.620
High resolution limit [Å]	1.530	4.560	1.530
Rmerge	0.084	0.057	1.734
Rmeas	0.113	0.075	2.430
Number of reflections	51075	815	2010
<I/σ(I)>	3.94
Completeness [%]	40.9
Redundancy	5.636
CC(1/2)	0.988	0.984	0.169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M magnesium chloride hexahydrate; 0.1M tris hydrochloride pH 8.5; 30% (w/v) PEG 4000