12FC

Crystal structure of unliganded Fab 7118

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-20
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	44.665, 73.202, 150.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.380 - 2.290
R-factor	0.2234
Rwork	0.218
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.290	2.290
Number of reflections	21869	1129
<I/σ(I)>	43.9
Completeness [%]	95.0
Redundancy	11.7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Potassium Formate, 20% (w/v)PEG 3350. 10% (v/v) ethylene glycol