12FA
Crystal structure of EsxA from Streptococcus intermedius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92009 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.463, 22.549, 61.300 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 25.450 - 1.480 |
| R-factor | 0.2079 |
| Rwork | 0.207 |
| R-free | 0.22650 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.549 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.450 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.073 | 0.633 |
| Number of reflections | 14382 | 1362 |
| <I/σ(I)> | 14.73 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Bis-Tris:HCl pH 5.5, and 2 M ammonium sulfate |
