12AW

Response regulator domains of BT4124 Hybrid Two-Component System from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 63
Unit cell lengths	132.218, 132.218, 70.243
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.170 - 2.900
R-factor	0.2394
Rwork	0.232
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	3.070
High resolution limit [Å]	2.900	2.900
Rmerge	0.121	1.168
Number of reflections	15725	2512
<I/σ(I)>	14.2	1.8
Completeness [%]	99.7	98.4
Redundancy	9.8	7.7
CC(1/2)	0.998	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M DL-Malic Acid, pH 7.0 20% PEG 3350