126D
CRYSTAL STRUCTURE OF CATGGCCATG AND ITS IMPLICATIONS FOR A-TRACT BENDING MODELS
Experimental procedure
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.600, 42.490, 34.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.196 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.053 * |
| Number of reflections | 3217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusionstanding drops * | 5 * | VAPOR DIFFUSION, SITTING DROP, temperature 278.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | decamer | 0.54 (mM) | double helix |
| 2 | 1 | drop | 145 (mM) | ||
| 3 | 1 | drop | spermine tetrahydrochloride | 4.5 (mM) | |
| 4 | 1 | drop | MPD | 15 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 45 (%) |
