11ZT

Kindlin-3/Integrin Beta2 Cytoplasmic Tail Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	90.256, 102.976, 114.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.453 - 2.397
R-factor	0.22346191161
Rwork	0.222
R-free	0.25466
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.457	2.490
High resolution limit [Å]	2.397	2.400
Rmerge	0.055
Number of reflections	41545	4019
<I/σ(I)>	22.54
Completeness [%]	97.5
Redundancy	7.2
CC(1/2)	1.000	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M sodium thiocyanate, 10% PEG3350