11OX

Crystal Structure of Honey Truffle Active Component 1 through 4 with glucose bound (Monoclinic P form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-12
Detector	Bruker PHOTON III
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	45.638, 44.472, 63.748
Unit cell angles	90.00, 106.68, 90.00

Refinement procedure

Resolution	20.890 - 1.700
R-factor	0.1911
Rwork	0.189
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.019
Data reduction software	SAINT
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.0_5750: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.170	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.053	1.566
Rmeas	0.055	1.680
Rpim	0.014	0.602
Total number of observations	370664	10431
Number of reflections	26884	1378
<I/σ(I)>	28.3	1.6
Completeness [%]	99.1
Redundancy	13.8	7.6
CC(1/2)	1.000	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	12.5% (v/v) MPD, 12.5% PEG 1000, 12.5% (w/v) PEG 3350, 0.05M Sodium HEPES, 0.05M MOPS pH 7.5, 0.02 M D-Glucose, 0.02 M D-Mannose, 0.02 M D-Galactose, 0.02 M L-Fucose, 0.02 M D-Xylose, 0.02 M N-Acetyl-D-Glucosamine, 0.5 mM Manganese(II) chloride tetrahydrate, 0.5 mM Cobalt chloride hexahydrate, 0.5 mM Nickel chloride hexahydrate, 0.5 mM Zinc acetate dihydrate