11KP

Crystal structure of the Caenorhabditis elegans telomeric POT-1-TEBP-1 complex interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-27
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97158
Spacegroup name	I 1 2 1
Unit cell lengths	73.799, 39.810, 103.941
Unit cell angles	90.00, 97.03, 90.00

Refinement procedure

Resolution	51.580 - 1.480
R-factor	0.1847
Rwork	0.183
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.830
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2-5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.500	1.500
High resolution limit [Å]	1.470	1.470
Rmerge	0.085	0.603
Number of reflections	94669	4657
<I/σ(I)>	10.2
Completeness [%]	96.5
Redundancy	6.7
CC(1/2)	0.997	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.75	289	SeMet-substituted complex (17 mg/ml) was mixed 1:1 with the well solution 100 mM MES (pH 6.75), 100 mM NaCl, and 8% PEG 3350. Crystals were harvested in 20 mM Tris (pH 8.0), 100 mM MES (pH 6.5), 200 mM NaCl, 10% PEG 3350, and cryoprotected in harvesting solution with 35% ethylene glycol