11KK
Crystal structure of HerA Like Helicae YjgR with NTPase fold from Escherchia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-01 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.645, 229.362, 99.531 |
| Unit cell angles | 90.00, 112.88, 90.00 |
Refinement procedure
| Resolution | 47.040 - 2.900 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.172 | 1.208 |
| Rmeas | 0.197 | 1.427 |
| Rpim | 0.094 | 0.746 |
| Number of reflections | 78317 | 3511 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 4.1 | 3.4 |
| CC(1/2) | 0.961 | 0.349 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.15 M D/L-Malic acid pH 7.0, 20 % (w/v) PEG 3350 |
