11HB

Crystal structure of a GII.4 norovirus capsid P domain in complex with neutralizing antibody 17A5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97648
Spacegroup name	C 1 2 1
Unit cell lengths	271.954, 134.910, 133.913
Unit cell angles	90.00, 91.75, 90.00

Refinement procedure

Resolution	48.430 - 3.360
R-factor	0.2598
Rwork	0.258
R-free	0.29960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	3.440
High resolution limit [Å]	3.360	3.360
Number of reflections	68516	68516
<I/σ(I)>	7.4
Completeness [%]	98.4
Redundancy	6.9
CC(1/2)	0.982	0.415

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	297.15	0.1M Sodium Acetate, 2M Sodium formate