11HA
Crystal structure of a GII.4 norovirus capsid P domain in complex with neutralizing antibody 24C10
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.117, 56.428, 182.117 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.700 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.684 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.780 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.146 | |
| Number of reflections | 49114 | 3474 |
| <I/σ(I)> | 12.22 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.998 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297.15 | 0.2 M HEPES: NaOH, pH 7.5, 8 % (w/v) PEG 8000, 8 % (v/v) Ethylene Glycol |
