11CT
Crystal structure of mouse IgG2a-Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.248, 98.136, 61.922 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 66.690 - 3.210 |
| R-factor | 0.2415 |
| Rwork | 0.239 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.694 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.050 | 3.270 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.176 | 1.070 |
| Rmeas | 0.209 | 1.271 |
| Rpim | 0.112 | 0.680 |
| Total number of observations | 44125 | 2095 |
| Number of reflections | 13104 | 621 |
| <I/σ(I)> | 11.4 | 1.3 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.975 | 0.320 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295.15 | 0.2M Bis-Tris, pH 6.0, 0.2M Lithium Sulfate, 20% PEG3350 |
