11AP

Crystal structure of mouse DXO in complex with the UDP-N-acetylglucosamine cap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97905
Spacegroup name	P 1 21 1
Unit cell lengths	49.880, 87.990, 53.580
Unit cell angles	90.00, 113.03, 90.00

Refinement procedure

Resolution	49.310 - 1.800
R-factor	0.167
Rwork	0.165
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	38997	6193
<I/σ(I)>	11.8
Completeness [%]	98.2
Redundancy	3.9
CC(1/2)	0.995	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293.15	21-25% (w/v) PEG 3350 and 0.1 M Tris (pH 6.8).