118D
CRYSTAL AND MOLECULAR STRUCTURE OF D(GTGCGCAC): INVESTIGATION OF THE EFFECTS OF BASE SEQUENCE ON THE CONFORMATION OF OCTAMER DUPLEXES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 263 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.220, 42.220, 25.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.640 |
| R-factor | 0.154 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.017 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.640 |
| Rmerge | 0.035 * |
| Total number of observations | 11665 * |
| Number of reflections | 2767 |
| Completeness [%] | 91.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | octamer duplex | 2 (mM) | |
| 2 | 1 | drop | 3 (mM) | ||
| 3 | 1 | drop | spermine | 2 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 5 | 1 | reservoir | MPD | 3.0 (%) |
