116D
CRYSTAL AND MOLECULAR STRUCTURE OF THE A-DNA DODECAMER D(CCGTACGTACGG): CHOICE OF FRAGMENT HELICAL AXIS
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | OSCILLATION CAMERA |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.200, 46.200, 71.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.15 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.038 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.040 * |
| Total number of observations | 4971 * |
| Number of reflections | 1664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 4 * | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | [CO(NH3)6]3+ | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 3.5 (mM) | |
| 2 | 1 | drop | cobalt hexamine | 12 (mM) | |
| 3 | 1 | drop | spermine | 5 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 10 (mM) |
