10ZG

Novobiocin Glycosyltransferase (Ngt-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	80
Detector technology	PIXEL
Collection date	2025-06-11
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	54.029, 95.219, 102.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.010 - 2.000
R-factor	0.1756
Rwork	0.174
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.909
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.010	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.282
Rmeas	0.062	0.316
Rpim	0.028	0.138
Number of reflections	36540	3573
<I/σ(I)>	21	4.4
Completeness [%]	99.9
Redundancy	4.8
CC(1/2)	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.16 mM Magnesium acetate, 0.08 mM Na-cacodylate pH 6.7, 16% PEG 8000 (w/v) and 10% glycerol (v/v)