10YZ

Crystal structure of mouse DXO in complex with pGGGUU RNA and two magnesium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	46.365, 87.999, 50.156
Unit cell angles	90.00, 114.37, 90.00

Refinement procedure

Resolution	42.230 - 1.600
R-factor	0.1845
Rwork	0.183
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((2.0_5936: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	1.700
High resolution limit [Å]	1.600	4.780	1.600
Rmerge	0.017	0.012	0.123
Rmeas	0.023	0.017	0.174
Number of reflections	90199	537	2883
<I/σ(I)>	12.12
Completeness [%]	94.8
Redundancy	2
CC(1/2)	0.999	0.999	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 3350