10YV

Structure of a putative GH97 from Cellulomonas fimi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 VENTURE
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-17
Detector	Bruker PHOTON II
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	71.424, 74.434, 132.431
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.970 - 2.150
R-factor	0.2086
Rwork	0.207
R-free	0.23590
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	SAINT
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.220	2.170
High resolution limit [Å]	2.150	2.150
Number of reflections	73531	1266
<I/σ(I)>	2
Completeness [%]	99.2	99.84
Redundancy	7.1
CC(1/2)	0.950	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Cadmium chloride hydrate, 0.1 M Sodium acetate trihydrate pH 4.6 and 30% v/v Polyethylene glycol 400