10YO
Nocardia pseudobrasiliensis DnaA DI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-18 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 100.342, 100.342, 100.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.480 - 2.800 |
| R-factor | 0.2192 |
| Rwork | 0.216 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.578 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.480 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.099 | 0.824 |
| Rpim | 0.019 | 0.159 |
| Number of reflections | 4253 | 605 |
| <I/σ(I)> | 32.4 | 3.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 25.8 | 26.4 |
| CC(1/2) | 1.000 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.8 M sodium phosphate monobasic monohydrate; potassium phosphate dibasic, pH 5.0 |
